The Myc Gene book Methods and Protocols Methods in Molecular Biology, Vol. 1012 Soucek, Laura, Sodir, Nicole M. (Eds.)
The Myc Gene book Methods and Protocols Methods in Molecular Biology, Vol. 1012 Soucek, Laura, Sodir, Nicole M. (Eds.)
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The Myc Gene
Methods and Protocols
Series: Methods in Molecular Biology, Vol. 1012
Soucek, Laura, Sodir, Nicole M. (Eds.)
2013, XI, 282 p. 44 illus., 29 illus. in color.
A product of Humana Press
- Features tips on trouble-shooting and avoiding known pitfalls
- Contains key notes and implementation advice from the experts
Myc controls multiple cellular functions,
including cell proliferation, growth, differentiation and death, both
directly and indirectly, through its modulation of downstream
transcriptional programs. In The Myc Gene: Methods and Protocols,
experts in the field summarize the standard and novel techniques that
allow the studying of Myc mechanism of action in normal and cancer
cells, in vitro and in vivo, in one succinct manual. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting and
avoiding known pitfalls.
Content Level » Professional/practitioner
Keywords » Myc - Myc mechanism - cancer - cancer therapy - cellular functions - in vitro and in vivo
Table of contents
1. The Myc World Within Reach
Nicole M. Sodir and Laura Soucek
2. Methods for the Expression, Purification, Preparation and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZs
Marie-Eve Beaulieu, François-Olivier Mc Duff, Mikaël Bédard, Martin Montagne and Pierre Lavigne
3. Analyzing Myc in Cell Transformation and Evolution
Markus Hartl and Klaus Bister
4. Identifying Myc Interactors
Romina Ponzielli, William B. Tu, Igor Jurisica, and Linda Z. Penn
5. Detection of c-Myc Protein-Protein Interactions And Phosphorylation Status by Immunoprecipitation
Colin J. Daniel, Xiaoli Zhang and Rosalie C. Sears
6. An Efficient Way of Studying Protein–Protein Interactions Involving HIF-α, c-Myc, and Sp1
Kenneth K.-W. To and L. Eric Huang
7. Methods for Determining Myc-induced Apoptosis
Dan Lu and Trevor D. Littlewood
8. Methods to Study Myc-regulated Cellular Senescence
Vedrana Tabor, Matteo Bocci and Lars-Gunnar Larsson
9. Chromatin Immunoprecipitation Assays for Myc and N-Myc
Bonnie L. Barrilleaux, Rebecca Cotterman, and Paul S. Knoepfler
10. Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene RepressionRui Song, Nicole Sponer, and Lin He
11. Genome-wide Analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing
Rene Jackstadt, Antje Menssen and Heiko Hermeking
12. A High Throughput siRNA Screening Platform to Identify MYC Synthetic Lethal Genes as Candidate Therapeutic Targets
Carla Grandori
13. Investigating Myc-dependent Translational Regulation in Normal and Cancer Cells
John T. Cunningham, Michael Pourdehnad, Craig R. Stumpf, and Davide Ruggero
14. Studying Myc’s Role in Metabolism Regulation
Anne Le and Chi V. Dang
15. Generation of a Tetracycline Regulated Mouse Model of MYC-induced T-cell Acute Lymphoblastic Leukemia
Kavya Rakhra and Dean W. Felsher
16. Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration and Tumourigenesis
David J Huels, Patrizia Cammareri, Rachel A Ridgway, Jan P Medema and Owen J Sansom
17. Detection of c-myc Amplification in Formalin-fixed Paraffin-Embedded Tumor Tissue by Chromogenic in situ Hybridization (CIHS)
Natasa Todorovic-Rakovic
18. Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules
Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov
Nicole M. Sodir and Laura Soucek
2. Methods for the Expression, Purification, Preparation and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZs
Marie-Eve Beaulieu, François-Olivier Mc Duff, Mikaël Bédard, Martin Montagne and Pierre Lavigne
3. Analyzing Myc in Cell Transformation and Evolution
Markus Hartl and Klaus Bister
4. Identifying Myc Interactors
Romina Ponzielli, William B. Tu, Igor Jurisica, and Linda Z. Penn
5. Detection of c-Myc Protein-Protein Interactions And Phosphorylation Status by Immunoprecipitation
Colin J. Daniel, Xiaoli Zhang and Rosalie C. Sears
6. An Efficient Way of Studying Protein–Protein Interactions Involving HIF-α, c-Myc, and Sp1
Kenneth K.-W. To and L. Eric Huang
7. Methods for Determining Myc-induced Apoptosis
Dan Lu and Trevor D. Littlewood
8. Methods to Study Myc-regulated Cellular Senescence
Vedrana Tabor, Matteo Bocci and Lars-Gunnar Larsson
9. Chromatin Immunoprecipitation Assays for Myc and N-Myc
Bonnie L. Barrilleaux, Rebecca Cotterman, and Paul S. Knoepfler
10. Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene RepressionRui Song, Nicole Sponer, and Lin He
11. Genome-wide Analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing
Rene Jackstadt, Antje Menssen and Heiko Hermeking
12. A High Throughput siRNA Screening Platform to Identify MYC Synthetic Lethal Genes as Candidate Therapeutic Targets
Carla Grandori
13. Investigating Myc-dependent Translational Regulation in Normal and Cancer Cells
John T. Cunningham, Michael Pourdehnad, Craig R. Stumpf, and Davide Ruggero
14. Studying Myc’s Role in Metabolism Regulation
Anne Le and Chi V. Dang
15. Generation of a Tetracycline Regulated Mouse Model of MYC-induced T-cell Acute Lymphoblastic Leukemia
Kavya Rakhra and Dean W. Felsher
16. Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration and Tumourigenesis
David J Huels, Patrizia Cammareri, Rachel A Ridgway, Jan P Medema and Owen J Sansom
17. Detection of c-myc Amplification in Formalin-fixed Paraffin-Embedded Tumor Tissue by Chromogenic in situ Hybridization (CIHS)
Natasa Todorovic-Rakovic
18. Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules
Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov
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